Nebiocalculator Dna Calculator

DNA Concentration Formula:

\[ nM = \frac{ng/\mu L \times 10^6}{660 \times length} \]

DNA Concentration (nM):

Unit Converter ▲

Unit Converter ▼

From:	To:

1. What is the DNA Concentration Calculator?

The DNA Concentration Calculator converts DNA concentration from ng/μL to nM using the standard molecular biology formula. This conversion is essential for accurate molar calculations in molecular biology experiments.

2. How Does the Calculator Work?

The calculator uses the DNA concentration formula:

\[ nM = \frac{ng/\mu L \times 10^6}{660 \times length} \]

Where:

\( ng/\mu L \) — DNA concentration in nanograms per microliter
\( length \) — DNA length in base pairs (bp)
\( 660 \) — Average molecular weight of one DNA base pair (g/mol)
\( 10^6 \) — Conversion factor for ng to g and μL to L

Explanation: The formula converts mass concentration to molar concentration by accounting for the molecular weight of DNA and the specific length of the DNA fragment.

3. Importance of DNA Concentration Calculation

Details: Accurate molar concentration calculations are crucial for PCR, sequencing, cloning, and other molecular biology techniques where precise DNA amounts are required for successful experiments.

4. Using the Calculator

Tips: Enter DNA concentration in ng/μL and DNA length in base pairs. Both values must be positive numbers (concentration > 0, length ≥ 1).

5. Frequently Asked Questions (FAQ)

Q1: Why is 660 used in the formula?
A: 660 g/mol is the average molecular weight of one DNA base pair, which accounts for the different molecular weights of A-T and G-C base pairs.

Q2: Can this calculator be used for RNA?
A: No, this calculator is specifically for DNA. RNA calculations use a different average molecular weight (approximately 340 g/mol per base).

Q3: What if I have double-stranded vs single-stranded DNA?
A: This formula is designed for double-stranded DNA. For single-stranded DNA, use 330 g/mol as the average molecular weight per base.

Q4: Why convert to molar concentration?
A: Molar concentration (nM) allows for more accurate calculations when working with enzymes, primers, and other reagents that interact with DNA on a molecular basis.

Q5: Are there limitations to this calculation?
A: The calculation assumes average base composition. For extremely GC-rich or AT-rich sequences, the actual molecular weight may vary slightly.