1. What is Oligo Molarity?

Oligo molarity refers to the concentration of oligonucleotides in solution, expressed in moles per liter (mol/L). It is a crucial parameter in molecular biology experiments involving DNA/RNA oligonucleotides.

2. How Does the Calculator Work?

The calculator uses the molarity formula:

Where:

• \( M \) — Molarity (mol/L)
• \( mass \) — Mass of oligo in grams
• \( MW \) — Molecular weight in g/mol
• \( volume \) — Volume of solution in liters

Explanation: The formula calculates the number of moles of oligo per liter of solution, providing the concentration needed for various molecular biology applications.

3. Importance of Molarity Calculation

Details: Accurate molarity calculation is essential for preparing oligonucleotide solutions with precise concentrations required for PCR, sequencing, hybridization, and other molecular techniques.

4. Using the Calculator

Tips: Enter mass in grams, molecular weight in g/mol, and volume in liters. All values must be positive numbers greater than zero.

5. Frequently Asked Questions (FAQ)

Q1: Why is accurate oligo molarity important?
A: Precise molarity ensures consistent results in experiments like PCR where oligo concentration affects amplification efficiency and specificity.

Q2: How do I determine molecular weight?
A: Molecular weight can be calculated based on nucleotide sequence or provided by the oligo synthesis company.

Q3: Can I use different volume units?
A: Yes, but ensure all units are consistent. Convert microliters to liters (divide by 1,000,000) before calculation.

Q4: What is a typical oligo concentration?
A: Working concentrations typically range from 10 μM to 100 μM, depending on the application.

Q5: How should I store oligo solutions?
A: Store at -20°C in aliquots to avoid repeated freeze-thaw cycles and degradation.